The star has given the entirety of his £25k award cash to the trust for venues, which operates to save the Britain's grassroots live music spots.
He was given the cheque on mid-October as his third album People Watching was chosen as the outstanding release of the last 12 months.
Fender has chosen to donate the funds to the MVT, in recognition of the vital role small stages played in his initial steps.
"As an artist wouldn't be doing what I am doing today if it weren't for all the shows I performed around the region, and beyond, when I was just getting going," said Fender. "These locations are renowned, but they are struggling."
Since the start of 2023, more than 150 of these locations have permanently closed – roughly 16% of the whole industry in the UK.
In the last year, major artists such as Pulp, Coldplay, Katy Perry and Ed Sheeran have all supported the Music Venues Trust in its attempts to support the industry, by applying a small levy to their ticket costs, which supports local stages.
Fender also joined in, raising in excess of £100,000 on his recent tour to help 38 local spots across England, Scotland, and Wales.
The donation assisted venues that were confronting potential shutdown due to challenges arising from flooding, fire incidents and family tragedies, as well as regulatory hurdles, court cases and sound issues.
Some locations were granted funding support in improving infrastructure and gear that helped artists and audiences.
"The idea that funds from shows in big venues helps the smaller venues, where it all starts for performers like me, is just logical," Sam Fender has commented.
The musician is among several artists to donate his award funds to good deeds.
When Pulp earned the award in the year 1996 for their release their work, frontman Cocker announced that the group would give their cash to the organization War Child.
In 2002, musician the artist divided her prize between a few organizations, such as the children's charity and a Sickle Cell charity.
"Additionally, I donated a £1,000 to Highgate Newtown, to their classes, because I trained in gymnastics when I was a child and they lacked better apparatus," she told outlets.
In recent years, the band contributed their winnings to the community center that developed their act, as well as other local music groups.
Also the 1994 recipients they donated their prize to a multiple sclerosis charity after a acquaintance was diagnosed the illness.
"Winning was sufficient," remarked vocalist Heather Small. "The money was the cherry on top but we didn't need the cherry, because we had the honor. So our success impacted another person's life."
In the past week, the Music Venues Trust shared it had rescued several grassroots venues in that region, by bringing them into public stewardship.
One venue in Southampton and the other in Bristol were purchased under the public acquisition scheme, which is funded by Arts Council England and enthusiasts who purchase "ownership units" in the buildings.